Aspergillus oryzae introduced with multi-copy genes

Extremely high production level of target protein by fermentation (3 g/L)

Overview

　In protein production using Aspergillus oryzae, the introduction of multi-copy genes encoding target proteins can be used as a means of improving productivity. However, if the DNA fragment to be introduced is tandemly inserted at one chromosomal locus and the repair through homologous recombination is repeated, the sequence may be excised, and the production cannot be increased as a result.
　Dr. Zhang and colleagues at the Graduate School of Agriculture, Tohoku University have developed a method to introduce DNA fragments into multiple loci of the chromosome of A. oryzae in a single transformation, and succeeded in obtaining a new A. oryzae in a short period of time. The A. oryzae of the present invention has been confirmed to have improved material productivity. Furthermore, by using a highly dispersible strain of mycelium as a host, it is expected to be low in viscosity when cultured in a fermenter, enabling continuous culture.

Since the patent application has not been published, the details of the gene transfer method are not shown. Please feel free to contact us as we can disclose this information after signing a non-disclosure agreement.

Secretory production of xylanase in liquid culture (mg/L)

Experiments were conducted to confirm the production of xylanase in Aspergillus oryzae with 1 copy or 13 copies cultured in YPD and YPM media. It was confirmed that the production of xylanase in A. oryzae with 13 copies increased about 10 times compared with that with 1 copy in YPM medium.

Product Application

・Industrial fermentation production of functional proteins and peptides such as enzymes
・Industrial fermentation production of bioactive low-molecular compounds such as amino acids and antibiotics

IP Data

IP No.　 ：PCT/JP2025/005036　
Inventor ：ZHANG Silai, SAGARA Sota, GOMI Katsuya, SHINTANI Takahiro
keyword ：Aspergillus oryzae, filamentous fungi, fermentation, improved productivity, introduce multi-copy genes